Project abstract for group ohlendor

Structural Analysis of Macromolecules

The goal of these studies is to understand the structural basis of how macromolecules function. The current focus is on two groups of proteins: dixoygenases that use metal ions to cleave aromatic rings and proteins from gram-positive pathogens. Examples of dioxygenases are protocatechuate 3,4-dioxygenase (PCD), homoprotocatechuate 2,3-dioxygenase (HPCD), and 1,2-catechol dioxygenase (CTD). Examples of proteins from gram-positive pathogens are pyrogenic toxin superantigens (PTSAgs), exfoliative toxins (ETs), streptococcal C5a protease (SCPB), beta-toxin from S. aureus, and aggregation substance and the pheromone response protein PrgX from Enterococcus faecalis.

The researchers use resources at MSI to refine structures of substrate and inhibitor complexes of mutants of PCD, HPCD, and CTD, and to solve and refine the structures of several proteins from pathogens.