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Project abstract for group tongcb
Apple and Potato Sequencing
This research program has two main foci: understanding limitations in accumulation of anthocyanins, the pigments responsible for the color of red potato tuber periderm (peel), and determining the biochemical and genetic bases behind "Honeycrisp" apple fruit crispness retention. As one way to study anthocyanins, the group utilizes high coverage DNA sequencing (DNAseq) to examine differences between "Norland," "Red Norland," and "Dark Red Norland" in order to determine the genetic changes that gave rise to the color sports. Bioinformatic analyses of these varieties, due to their tetraploid nature (there are potentially four possible sequence versions for any gene), is providing an intellectual challenge. "Honeycrisp" apple fruit have the unusual ability to maintain their crispness. The Tong lab has taken advantage of the University of Minnesota’s extensive collection of "Honeycrisp" offspring. They are extensively characterizing the textural phenotype of the offspring’s fruit during storage and use RNA sequencing to pinpoint genes responsible for crispness retention.
A bibliography of this group’s publications acknowledging MSI is attached.