Project abstract for group tretyako

Mass Spectrometry Based Proteomics Studies of DNA-Protein Cross-Links

Exposure to common antitumor drugs, environmental toxins, transition metals, UV light, ionizing radiation, and free radical-generating systems can result in cellular proteins becoming covalently trapped on DNA. These researchers employ mass spectrometry-based proteomics to investigate DNA-protein cross-links (DPCs) in human cells treated with clinically relevant concentrations of chemotherapeutic drugs (e.g., platinum compounds and nitrogen mustards) and metabolically activated carcinogens ( e.g., 1,2,3,4-diepoxybutane). More recent studies are focused on covalent DPCs that accumulate in brain and heart tissues as a result of exposure to endogenous reactive oxygen species, lipid peroxidation products, and transition metals. MSI access is required for processing the data obtained from mass spectrometry analysis. 

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