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Project abstract for group youngn
A Medicago truncatula Hapmap as a Platform for Exploring the Genetics of Symbiosis
This project uses whole-genome association mapping (WGAM) to create a Medicago HapMap as a framework for discovering the genes and gene-systems that play a role in the contemporary evolution of symbiosis. In brief, approximately 400 diverse Medicago lines will be resequenced using next generation sequencing technology in order to search for single nucleotide polymorphisms (SNPs), gene copy number variation, and insertion/deletion polymorphisms among the different Medicago lines. This massive database of SNPs and sequence variants will enable the discovery of genome segments with shared ancestry (haplotypes), which can then be used for statistical and data-mining techniques in the discovery of genotype/phenotype relationships involved in symbiosis. Because of the exceedingly high level of variant density discovery made possible through next-generation sequencing, this GWAM strategy will approach single gene level resolution.
A bibliography of this group’s publications acknowledging MSI is attached.