Self-Guided PDF

Analyzing ChIP-Seq Data using Galaxy

This practical, hands-on tutorial is designed to give participants experience with ChIP-Seq data analysis using the Galaxy platform. The analysis in this tutorial is typical of experiments using ChIP-Seq data to identify transcription factor binding sites in eukaryotic, high quality genomes.

Quality Control of Illumina Data at the Command Line

Want to take your analysis to the next level? 

This hands-on tutorial will introduce new bioinformaticitions to the skills needed to start to build more complex analysis pipelines. This tutorial will take you though the steps needed to run standard quality control analysis on Illumina data using scripting to automate the analysis. 

In this tutorial you will be introduced to:

Analyze ChIP-Seq Data at the Command Line

This tutorial is paired with Analyzing ChIP-Seq Data using Galaxy and will take the user though the same steps but, using the command line versions of the tools used in the Galaxy environment. This tutorial will:

1. Provide a brief introduction to MSI systems.

2. Provide a very brief introduction to UNIX.

3. Take users step-by-step though the process needed to analyze ChIP-Seq data

4. Provide users with a basic PBS script to automate the mapping and peak calling.

5. Teach users how to edit and run the script to be used in the future.

Rna-Seq Analysis

The RNA-Seq analysis tutorials includes two lectures and two hands-on guided tutorials. The lecture materials cover the basics of differential expression analysis and touches on other RNA-seq topics such as transcriptome assembly. 

Topics Covered:

Quality Control of Illumina Data with Galaxy

If you are new to bioinformatics this is the best place to start.

This hands-on tutorial will help a new user understand how to use the the Galaxy platform to analyze NGS data by working though the quality control steps needed for Illumina sequencing data.

This tutorial will: