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Tutorial Details: Introduction to MaxQuant Software for Proteomics

Date: Thursday, February 25, 2010, 01:00 pm - 03:00 pm
Location: 585 Walter
Instructor(s): Rongxiao Sa, Department of Chemistry

MaxQuant is a suite of algorithms for analysis of high-resolution mass spectrometry (Orbitrap and FT) data. It can be used for protein identification for non-labeled samples and identification and quantification for SILAC-labeled samples. MaxQuant includes all steps needed in a computational proteomic platform except that it uses the Mascot search algorithm for peptide identification.

In brief, raw data acquired on Orbitrap is processed using MaxQuant’s “Quant” module. The processed data is further searched with Mascot. Eventually, the Mascot search output files are subjected to statistic analysis and protein grouping using the “Identify” module.

In the tutorial we will discuss the algorithm, the recommended experimental setup, and hardware requirements. We will also include a walk-through of how to use MaxQuant.

This tutorial will also be available in the form of a webinar through UMConnect. Please visit http://www.oit.umn.edu/umconnect/ to ensure that your system meets the minimum requirements necessary to participate in this webinar.

Level: Introductory
Prerequisites: Introductory knowledge of proteomics