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Tutorial Details: RNA-Seq Analysis In Galaxy

Date: Friday, March 25, 2011, 01:00 pm - 03:00 pm
Location: 575 Walter
Instructor(s): Wayne Xu, MSI

Galaxy ( is a bioinformatics workflow framework that provides a Web interface to command line bioinformatics tools and enables sharing of data and tools between users. Within this framework, biologists can carry out a variety of computational analyses including gene expression profiling or RNA-seq, the focus of this tutorial. RNA- seq is quickly supplanting microarray technologies as the method of choice for transcriptomics. Tutorial participants will learn about the distinct computational issues that arise in applications where the reference genome has been sequenced versus cases where no reference genome is available. Strategies and workflows will be analyzed for each case, and participants will receive hands-on experience using state-of-the- art algorithms within Galaxy to obtain normalized transcript abundance counts from the raw sequenced reads. For more information about galaxy, visit or view a Galaxy video tour at

Level: Introductory
Prerequisites: Basic knowledge of genomics and statistics