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Tutorial Details: RNA-Seq Module 2: From QC to Differential Gene Expression. Using TopHat and Cufflinks in Galaxy

Date: Tuesday, April 24, 2012, 01:00 pm - 03:00 pm
Location: 402 Walter
Instructor(s): Ying Zhang, MSI

This tutorial is a walk-through of an RNA-seq analysis pipeline that uses some of the most popular tools: TopHat, Cufflinks and Galaxy. Previous experience with Galaxy is not required; however, attendees are encouraged to attend the RNA-seq tutorial Module 1 first. After this tutorial, attendees will be able to analyze the RNA-seq data using TopHat and Cufflinks in Galaxy.

The tutorial provides hand-on experience with the RNA-seq analysis programs FastQC, Fastq Groomer, TopHat, and Cufflinks. Attendees will learn how to use the bioinformatics platform Galaxy to upload, download, and analyze their RNA-seq data. Background information on the analytic tools and the working platform will be discussed. Sample data will be used for attendees to familiarize themselves with the analysis pipeline. This tutorial will also provide information on MSI support available to attendees and will encourage collaboration between MSI and local researchers.

Level: Introductory
Prerequisites: None