This is a practical, hands-on tutorial designed to give participants experience with RNA-Seq data analysis using Tophat and Cufflinks in Galaxy. The analysis in this tutorial is typical of experiments in plant species with adequate quality genomes and genome annotation. Participants are expected to be familiar with next-generation sequence data and basic theory of RNA-Seq. Participants do not need previous experience with Tophat or Cufflinks.
Prerequisites include: GALAXY 101 or experience with GALAXY and the Galaxy RNA-Seq Lecture.