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Tutorial Registration: RNA-Seq Applied Tutorial - Analysis of Human Data Set

Date: Tuesday, October 16, 2012, 01:00 pm - 04:00 pm
Location: 575 Walter
Instructor(s): Kevin A Silverstein PhD, MSI, Ying Zhang, MSI, John R Garbe,

RNA-seq is rapidly supplanting microarray analysis as a means of comprehensively profiling gene expression due to rapidly decreasing costs and unparalleled gene isoform resolution capabilities. In the last two years, the Tuxedo Suite Tools (i.e., Bowtie, TopHat, Cufflinks and CummeRbund) have become standardized software packages to analyze this rich and complex data. The Galaxy bioinformatics framework provides an invaluable front end to these and many other analysis packages, enabling researchers without in-depth programming knowledge and command-line familiarity to carry out their own data analysis.

In this hands-on tutorial, participants will perform standard best-practices analysis with sample Human RNA-seq data from the Human BodyMap project to illustrate from start to finish the use of RNA-seq to replace microarrays. This will include sample QA/QC, mapping via TopHat, assessing mapping statistics, visualizing data in Trackster and IGV, computing differential expression via Cuffdiff, and doing some sample comparisons via PCA analysis and clustering.

Familiarity with using Galaxy is strongly recommended (

Seating is limited. Please note however that this tutorial will be presented online. See details below in RNA-Seq Applied Tutorial - Analysis of Human Data Set - WEBINAR.

Level: Intermediate
Prerequisites: Familiarity with using Galaxy is strongly recommended
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