This lecture will cover the basics of RNA-Seq experimental design and data quality assessment, followed by an overview of data analysis for the detection of differentally expressed genes. Specific subtopics include:
This hands-on tutorial will cover installation and use of the SMRT portal at MSI to analyze PacBio sequencing data. The basics of full genome assembly and transcript assembly will be covered. At the end of this tutorial, participants should be able to:
The RNA-Seq analysis tutorials includes two lectures and two hands-on guided tutorials. The lecture materials cover the basics of differential expression analysis and touches on other RNA-seq topics such as transcriptome assembly.
If you are new to bioinformatics this is the best place to start.
This hands-on tutorial will help a new user understand how to use the the Galaxy platform to analyze NGS data by working though the quality control steps needed for Illumina sequencing data.